Effect of interaction of magnetic nanoparticles of Fe3O4 and artesunate on apoptosis of K562 cells
نویسندگان
چکیده
The present study evaluated whether the magnetic nanoparticles of Fe(3)O(4) (MNPs-Fe(3)O(4)) could enhance the activity of artesunate (ART), and to explore its potential mechanisms. Cytotoxicity of the copolymer of ART with MNPs-Fe(3)O(4) on K562 cells was detected by MTT assay and the apoptosis rate of K562 cells was measured by flow cytometry. Protein expression levels of bcl-2, bax, bcl-rambo, caspase-3, and survivin in K562 cells were measured by Western blot. After being incubated with the copolymer of ART with MNPs-Fe(3)O(4) for 48 hours, the growth inhibition rate of K562 cells was significantly increased compared with that of K562 cells treated with ART alone (P < 0.05), and the apoptosis rate of K562 cells was increased significantly compared with that of K562 cells treated with ART alone, suggesting that MNPs-Fe(3)O(4) can enhance the activity of ART. Interestingly, the copolymer-induced cell death was attenuated by caspase inhibitor Z-VAD-FMK. Our results also showed that treatment with the copolymer of MNPs-Fe(3)O(4) and ART increased the expression of bcl-2, bax, bcl-rambo, and caspase-3 proteins, and decreased the expression of survivin protein in K562 cells compared with ART treatment alone. These results suggest that MNPs-Fe(3)O(4) can enhance ART-induced apoptosis, which may be related to the upregulation of bcl-rambo and downregulation of survivin.
منابع مشابه
The Effect of Magnetic Fe3O4 Nanoparticles on the Growth of Genetically Manipulated Bacterium, Pseudomonas aeruginosa (PTSOX4)
Background: Magnetite (Fe3O4) nanoparticles are currently one of the important and acceptable magnetic nanoparticles for biomedical applications. To use magnetite nanoparticles for bacteria cell separation, the surface of nanoparticles would be modified for immobilizing of nanoparticles on the surface of bacteria. Functionalization of magnetite nanoparticles is performed by different s...
متن کاملSynergistic effect of the combination of nanoparticulate Fe3O4 and Au with daunomycin on K562/A02 cells
In this study, we have explored the possibility of the combination of the high reactivity of nano Fe3O4 or Au nanoparticles and daunomycin, one of the most important antitumor drugs in the treatment of acute leukemia clinically, to inhibit MDR of K562/A02 cells. Initially, to determine whether the magnetic nanoparticle Fe3O4 and Au can facilitate the anticancer drug to reverse the resistance of...
متن کاملCuO nanoparticles induce cytotoxicity and apoptosis in human K562 cancer cell line via mitochondrial pathway, through reactive oxygen species and P53
Objective(s): This study focused on determining cytotoxic effects of copper oxide nanoparticles (CuO NPs) on chronic myeloid leukemia (CML) K562 cell line in a cell-specific manner and its possible mechanism of cell death. We investigated the cytotoxicity of CuO NPs against K562 cell line (cancerous cell) and peripheral blood mononuclear cell (normal cell). Materials and Methods: The toxicity w...
متن کاملEffect of dispersion state of the magnetic Fe3O4 nanoparticles on the thermal distribution in Hyperthermia
Introduction: Magnetic nanoparticle (MNP) hyperthermia is a promising cancer treatment approach. It is based on the evidence that by injecting MNPs such as Fe3O4 in the tumor and subjecting them to an alternating magnetic field, they release heat, generating temperatures up to 42°C that can kill cancer cells by apoptosis, usually with lowest damage to normal tissue. In previous...
متن کاملSynthesis and Characterization of a Novel Fe3O4-SiO2@Gold Core-Shell Biocompatible Magnetic Nanoparticles for Biological and Medical Applications
Objectives: The study of core-shell magnetic nanoparticles has a wide range of applications because of the unique combination of the nanoscale magnetic core and the functional shell. Characterization and application of one important class of core-shell magnetic nanoparticles (MNPs), i.e., iron oxide core (Fe3O4/γ-Fe2O3) with a silica shell and outer of gold (Fe3O4-SiO2@Gold (FSG)) in Boron Neut...
متن کامل